the use of immunoglobulin y (igy) instead of polyclonal antibodies prepared in mammals has been reported recently. these antibodies are found to have better properties in terms of specificity and ease of large scale production. in this study, proteins from egg yolk were extracted with acid and chloroform. the result of cellulose acetate electrophoresis at ph 9 showed some extra bands which were...

conventional treatment of naja naja oxiana (nno) envenoming requires large volumes of equine antivenin raised against nno crude venom. the poor efficiency of this antivenin is assumed to be due to the high molecular weight non-toxic proteins, a strong immunogen, present in the crude venom. these proteins cause depression of antibody formation against the low molecular weight toxic components of...

the aim of the present work was to purify cysteine protease inhibitors from rapeseed (brassica napus l.), with potential activity on digestive protease of colorado potato beetle (cpb), leptinotarsa decemlineata (say). ammonium sulfate precipitated proteinaceous fractions; 30, 50, 70, and 100% showed 39.07, 57.03, 51.47, and 22.44% inhibition on the fourth instar larval gut general protease acti...

INTRODUCTION The term Gel Chromatography refers to the process of separation of solutes caused by the passage of a solution through a column filled with a porous gel. The dissolved particles may enter the pores fully or partially or they may remain completely excluded. This results in a sorting out of the dissolved particles according to their size, so that the largest particles migrate with th...

Gel-filtration chromatography is a versatile method that permits the effective separation of biological molecules in high yield. This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical matrix types. Applications of the technique are described, with references to the scientific literature.